Skip to main content
. 2014 Aug 27;70(Pt 9):1272–1275. doi: 10.1107/S2053230X14017403

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 96-well MRC plate
Temperature (K) 289
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 50 mM Tris–HCl, 200 mM NaCl, 0.05% LDAO pH 7.5
Composition of reservoir solution 0.1 M HEPES, 15%(v/v) PEG 400, 0.2 M CaCl2 pH 7.0
Volume and ratio of drop 1 µl, 1:1
Volume of reservoir (µl) 60