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Gel-filtration profile of APHdr (marked by an arrow) using a Superdex 200 column with 20 mM Tris–HCl pH 8.0, 200 mM NaCl. The column was calibrated using proteins of standard molecular mass (Sigma; catalogue No. MWGF200-1KT). The observed molecular mass of APHdr is 265 kDa (marked by an arrow in the upper inset). A 15% SDS–PAGE of purified APHdr is shown in the lower inset; lane 1 contains standard molecular-mass markers (labelled in kDa) and lane 2 contains APHdr protein.
