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. 2014 Aug 27;70(Pt 9):1292–1295. doi: 10.1107/S2053230X14017944

Table 1. Macromolecule-production information.

Source organism D. radiodurans R1
DNA source Bacterial cells
Forward primer CGTGGATCCAACAATTCGGAAACCCCGGCC
Reverse primer CGTTGTACATTACAGCCAGCGTTCCAGCCAGG
Cloning vector None
Expression vector pST50Tr
Expression host E. coli BL21(DE3)pLysS
Complete amino-acid sequence of the construct produced MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGSNNSETPAPGPDSLLALAFPSDPQVSPDGKQVAFVLAQISEEDPAKPDKDFARPRYRSGLWLSEGGAARPLTHAETGRGDSAPRWSPDGQNLAFVRSAGEVKAALMLLPLKGGEARRVTHFKNGVSGPQWSPDGRFIAFTTTADTEDKRDERGEARVLTRPVYRANGADWLPERPAALWLYDVEADKLREWYAPEIGIGALSWWPDSRGVLIVQSEDEWQASQWRQDVYDLPLPTADAPAAPQKLLDWNSAAHGLAPHPDGQRFALIGRPAGKGNTEHAHLYLIENGQHRRLDTGHDHPVGDAVGGDCHVGAFPEGPRWLDGDTLLFSSTVRGSVGLFTAHIGGGVKAYDHDPQGVISAFTANEHGVALIRESATRFPEVELNGQRVTDLHARFPFPVREPQRVTFETELGEGEGWVLLPEGEQKVPALLNIHGGPHTDYGHGFTHEFQLMAARGYGVCYSNPRGSVGYGQAWVDAIYGRWGTVDADDLLNFFDRCLEAVPRLDAAKTAVMGGSYGGFMTNWITGHTTRFQAAITDRCISNLISFGGTSDIGLRFWDDELGLDFSRRADALKLWDLSPLQYVENVKTPTLIVHSVLDHRCPVEQAEQWYAALHKHQVPVRFVRFPEENHELSRSGRPDRRLTRLNEYFAWLERWL