FIG. 5.

Nucleotide and deduced amino acid (one-letter notation) sequences of ksbA. The nucleotide numbering begins at the 5′-most ApaI site of the 1,885-bp ApaI fragment in the cloned 7-kb BamHI fragment. An asterisk denotes a translational stop codon. A probable ribosome-binding sequence, GGAGT (nucleotides 643 to 647), is present six nucleotides upstream of the putative ATG start codon (boxed). Underlining indicates the deleted nucleotide sequences of ksbA for construction of the ksbA deletion mutant. The regions amplified by the initial PCR with the designed primers are shown by upper dotted lines.