FIG. 6.

Growth curves (A) and bafilomycin B₁ production (B) in liquid culture of a wild-type strain (solid circles), a ksbA disruptant (strain RD1) (solid squares), and a ksbA-complemented strain (strain RDC1) (solid triangles). Each strain was grown at 28°C in ISP2 medium. The amounts of bafilomycin B₁ at the indicated times were measured as described in Materials and Methods. Growth was monitored by measuring the optical density at 600 nm (OD₆₀₀). Three independent ksbA disruptants and ksbA-complemented strains showed an identical pattern; only the representative data on strain RD1 and RDC1 are shown.