TABLE 1.
Autoregulator-binding activity of rKsbA and native KsbA
| Nonlabeled autoregulator | 3H-labeled autoregulator | Specific binding activity (pmol/mg)a with nonlabeled autoregulator at the indicated concn (μM)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| pUC19, 125 | pUC19 + ksbA
|
K. setae
|
|||||||
| 125 | 12.5 | 1.25 | 0.125 | 0.0125 | 125 | 12.5 | |||
| SCB1 | [3H]SCB1 | 0 | 0.168 | 0.131 | 0.058 | 0.042 | 0.018 | 0.069 | 0.049 |
| IM-2-C5 | [3H]IM-2-C5 | 0 | 0.021 | 0 | —b | — | — | 0.008 | 0 |
| VB-C6 | [3H]VB-C7 | 0 | 0.032 | 0 | — | — | — | 0.015 | 0 |
| A-factor | [3H]SCB1 | 0 | 0.159 | 0.035 | — | — | — | 0.058 | 0 |
a
Values are averages from three independent experiments.
b
—, not determined.