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. 2014 Aug 29;70(Pt 9):2390–2400. doi: 10.1107/S1399004714014370

Table 2. Comparison between data sets from crystals harvested with the Crystal Catcher and Kapton meshes.

Data from glucose isomerase and thermolysin crystals mounted with the two supports were collected both at room temperature (293 K) and at cryo-temperature (100 K). Values in parentheses are for the highest resolution shell.

  Glucose isomerase Thermolysin
Relative humidity (%) 94.00 97.00
Holder/adhesive CT-100/‘Type A’ Mesh CT-200/‘Type B’ Mesh
Temperature (K) 293 100 293 100 293 100 293 100
Space group I222 I222 I222 I222 P6122 P6122 P6122 P6122
a (Å) 94.14 92.99 94.16 92.99 93.67 93.04 93.70 93.15
b (Å) 99.37 98.68 99.38 98.33 93.67 93.04 93.70 93.15
c (Å) 102.94 102.69 102.96 102.64 130.36 127.56 130.63 128.31
α = β (°) 90 90 90 90 90 90 90 90
γ (°) 90 90 90 90 120 120 120 120
Mosaicity (°) 0.142 0.139 0.075 0.084 0.035 0.176 0.017 0.182
Resolution (Å) 40.4–2.0 (2.1–2.0) 40.1–2.0 (2.1–2.0) 39.3–2.0 (2.1–2.0) 40.0–2.0 (2.1–2.0) 46.8–1.8 (1.9–1.8) 46.5–3.2 (3.3–3.2) 46.9–1.8 (1.9–1.8) 64.2–3.2 (3.3–3.2)
Completeness (%) 99.1 (99.6) 99.1 (98.8) 99.7 (99.1) 99.5 (98.3) 97.7 (98.5) 95.5 (96.1) 100.0 (99.9) 100.0 (100.0)
Multiplicity 3.7 (3.8) 3.6 (3.8) 3.7 (3.8) 3.7 (3.8) 11.0 (11.1) 10.7 (10.8) 10.7 (10.9) 10.1 (10.4)
I/σ(I)〉 25.9 (15.9) 29.5 (20.8) 39.1 (22.0) 30.8 (18.0) 18.0 (4.9) 17.3 (4.2) 12.1 (3.4) 12.5 (3.4)
R merge 0.053 (0.191) 0.034 (0.058) 0.023 (0.051) 0.031 (0.066) 0.075 (0.385) 0.112 (0.518) 0.123 (0.506) 0.148 (0.634)
Wilson B factor (Å2) 12.10 11.34 16.40 10.80 20.53 74.49 14.57 67.70
Total observations 120868 (9130) 115163 (8727) 121574 (9127) 117979 (8726) 341635 (25171) 58058 (4220) 377399 (25186) 58939 (4294)
Total unique 32581 (2400) 31723 (2326) 32730 (2387) 31915 (2323) 30996 (2262) 5449 (389) 32092 (2318) 5845 (411)

R merge(I) = Inline graphic Inline graphic.