Table 2. Comparison between data sets from crystals harvested with the Crystal Catcher and Kapton meshes.
Data from glucose isomerase and thermolysin crystals mounted with the two supports were collected both at room temperature (293 K) and at cryo-temperature (100 K). Values in parentheses are for the highest resolution shell.
| Glucose isomerase | Thermolysin | |||||||
|---|---|---|---|---|---|---|---|---|
| Relative humidity (%) | 94.00 | 97.00 | ||||||
| Holder/adhesive | CT-100/‘Type A’ | Mesh | CT-200/‘Type B’ | Mesh | ||||
| Temperature (K) | 293 | 100 | 293 | 100 | 293 | 100 | 293 | 100 |
| Space group | I222 | I222 | I222 | I222 | P6122 | P6122 | P6122 | P6122 |
| a (Å) | 94.14 | 92.99 | 94.16 | 92.99 | 93.67 | 93.04 | 93.70 | 93.15 |
| b (Å) | 99.37 | 98.68 | 99.38 | 98.33 | 93.67 | 93.04 | 93.70 | 93.15 |
| c (Å) | 102.94 | 102.69 | 102.96 | 102.64 | 130.36 | 127.56 | 130.63 | 128.31 |
| α = β (°) | 90 | 90 | 90 | 90 | 90 | 90 | 90 | 90 |
| γ (°) | 90 | 90 | 90 | 90 | 120 | 120 | 120 | 120 |
| Mosaicity (°) | 0.142 | 0.139 | 0.075 | 0.084 | 0.035 | 0.176 | 0.017 | 0.182 |
| Resolution (Å) | 40.4–2.0 (2.1–2.0) | 40.1–2.0 (2.1–2.0) | 39.3–2.0 (2.1–2.0) | 40.0–2.0 (2.1–2.0) | 46.8–1.8 (1.9–1.8) | 46.5–3.2 (3.3–3.2) | 46.9–1.8 (1.9–1.8) | 64.2–3.2 (3.3–3.2) |
| Completeness (%) | 99.1 (99.6) | 99.1 (98.8) | 99.7 (99.1) | 99.5 (98.3) | 97.7 (98.5) | 95.5 (96.1) | 100.0 (99.9) | 100.0 (100.0) |
| Multiplicity | 3.7 (3.8) | 3.6 (3.8) | 3.7 (3.8) | 3.7 (3.8) | 11.0 (11.1) | 10.7 (10.8) | 10.7 (10.9) | 10.1 (10.4) |
| 〈I/σ(I)〉 | 25.9 (15.9) | 29.5 (20.8) | 39.1 (22.0) | 30.8 (18.0) | 18.0 (4.9) | 17.3 (4.2) | 12.1 (3.4) | 12.5 (3.4) |
| R merge † | 0.053 (0.191) | 0.034 (0.058) | 0.023 (0.051) | 0.031 (0.066) | 0.075 (0.385) | 0.112 (0.518) | 0.123 (0.506) | 0.148 (0.634) |
| Wilson B factor (Å2) | 12.10 | 11.34 | 16.40 | 10.80 | 20.53 | 74.49 | 14.57 | 67.70 |
| Total observations | 120868 (9130) | 115163 (8727) | 121574 (9127) | 117979 (8726) | 341635 (25171) | 58058 (4220) | 377399 (25186) | 58939 (4294) |
| Total unique | 32581 (2400) | 31723 (2326) | 32730 (2387) | 31915 (2323) | 30996 (2262) | 5449 (389) | 32092 (2318) | 5845 (411) |
R
merge(I) =
.