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The conserved putative motif of the nuclease domain of PvuRts1I that is involved in metal-ion binding and catalysis. (a) Sequence alignment of the putative motif of PvuRts1I homologues. Absolutely conserved amino acids are indicated by blue triangles. (b) The in vitro modification-dependent enzymatic activity of PvuRts1I and its mutants on 5-hmC.
