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Specificity for oriT in relaxosome formation. Whole-cell lysates were analyzed by gel electrophoresis. Plasmids are shown as follows: lane 1, pC221; lane 2, pC221mobA4; lane 3, pC223; lane 4, pC221cop903; lane 5, pC223oriT1; lane 6, pC223oriT2; lane 7, pC221oriT3; and lane 8, pC221oriT4. CHR, chromosomal DNA; OC and SC, open circular and negatively supercoiled plasmid DNA, respectively. Loadings have been normalized to compensate for the higher copy number of the pC221cop903 replicon.
