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Schematic diagram of clones and subclones. The colony color (blue or white) of E. coli DH5αF′-145 cells carrying each clone was tested on LB plates containing X-Gal, tetracycline, and ampicillin. The arrow indicates the glxR gene. The cloning vector pMT1 is not shown. Abbreviations: B, BamHI; E, EcoRI; K, KpnI; Sa, SalI; Sc, ScaI; Sp, SphI; X, XhoI; Xb, XbaI.
