FIG. 7.

Gel shift assay with purified GlxR. Purified GlxR proteins were incubated with the probe, and the mixtures were analyzed by 6% PAGE. (A) Schematic diagram of the aceB promoter region. (B) Gel shift in the absence of cAMP. (C) Gel shift in the presence of cAMP. cAMP was added (to 0.2 mM) to the assay mixture, gels, and PAGE buffer. Lane 1 contained no protein; lanes 2 through 8 contained 30 ng, 60 ng, 130 ng, 250 ng, 500 ng, 1.0 μg, and 2.0 μg of purified GlxR protein, respectively. Arrows indicate shifted bands.