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Illustrative PCR results for ETT2 gene cluster. Strains, from top to bottom: O157 Sakai strain (complete ETT2 gene set), ECOR1 (a B171-8-like strain with an 8.7-kb deletion), K-12 (14.6-kb deletion), and CFT073 (UPEC, with no ETT2). Lanes, from left to right: molecular weight markers, ∼5-kb amplicons obtained with ETT2 TP-PCR primer pairs 1 to 10 (see text for details), negative control (DNA, no primers), and molecular weight markers (HyperLadder I; Bioline).
