TABLE 2.
Primers used to detect ETT2 and eip gene clusters
| Primer | Annealing site | Sequence |
|---|---|---|
| ETT2-1F | ECs3693 | GACCCAGCGCACCTGAGTAAGT |
| ETT2-1R | ECs3697 | AAGAGCGCAGTGTTTTGCCTGT |
| ETT2-2F | ECs3696 | GTGTGTTACCTCCGGGTCATCC |
| ETT2-2R | ECs3701 | CGCCGGACGATTTAAAGATGAG |
| ETT2-3F | ECs3700 | CGCACTGTGGATGCTCTGTCTT |
| ETT2-3R | ECs3706 | CGACTCATGGATTTGCACCAGA |
| ETT2-4F | ECs3705 | AATGACCAGGGACGAGCAAATC |
| ETT2-4R | ECs3711 | TATCCATTGCAAAACCCGCATT |
| ETT2-5F | ECs3709 | ATGTGCCTAACCCGCTCAAAAA |
| ETT2-5R | Intergenic | ACCGACCCTGATCTGGTTGTAA |
| ETT2-6F | ECs3719 | GGGAAATTATCAGCAAGCCATGA |
| ETT2-6R | ECs3726 | GCAGAAGAGAGTGGCAGCTGGT |
| ETT2-7F | ECs3726 | AGCGCGCCATTTACACGTATCT |
| ETT2-7R | ECs3732 | TGCACTTGATGCGAGTTGTTCA |
| ETT2-8F | ECs3731 | GGTGGGCAATGGAATTATGAGC |
| ETT2-8R | ECs3737 | AAACAGCGGCAGAAACCCACTA |
| ETT2-9F | ECs3736 | TCGGTCACCTTTTTGCCAATCT |
| ETT2-9R | ECs3741 | TCCCGTTAATGGTGCATTCGAT |
| ETT2-10F | ECs3740 | AATTACGCCTGGCATTTGGTGT |
| ETT2-10R | ECs3744 | TCAGGCGAACGGTATCGTCATA |
| LEE-1F | ECs4602 | TTAAGGCATCGATGTGTCCTTC |
| LEE-1R | ECs4595 | GACATCTTGTTCTGCGCCATTA |
| LEE-2F | Intergenic | TTTTAAACTGCAGCGACCTTACC |
| LEE-2R | ECs4586 | GCCTGAGGATCTGTTTTTGCTT |
| LEE-3F | ECs4588 | CGGAACTCATCGAAAGGTGTTT |
| LEE-3R | ECs4579 | CAAAACAAACAAAAACGGAACG |
| LEE-4F | ECs4579 | GCATTATACGCACCAACTGCAT |
| LEE-4R | ECs4572 | CGACATCTTGCAACAATGAACA |
| LEE-5F | ECs4573 | CTGAATGACCGATGGTGCTAAG |
| LEE-5R | ECs4568 | CCCCATCGTGTACTCACCAATA |
| LEE-6F | ECs4568 | ACTTCCGCGATCAAGGTAAAAA |
| LEE-6R | ECs4559 | ACCAGGATTCGACTGCAGCTTA |
| LEE-7F | ECs4559 | CGACGATTTGGTCGTTGAATAA |
| LEE-7R | ECs4533 | CTCCCATGCCATAACCAATTTT |
| LEE-8F | ECs4533 | TTATCGGTCTCAGCACCCCTAT |
| LEE-8R | ECs4531 | CTTTCGCTCAATGATGTTCCTG |
| 8.7kb-F | ECs3726 | AGACCAGCTGCCACTCTCTTCT |
| 8.7kb-R | ECs3736 | GCTTGATTTAGGGGGAGAATCC |
| NoETT2-F | ygeG | CCTGATCGTGGGTATCCTGT |
| NoETT2-R | ygeR | GCTTGCATTTCCAGATTCGT |
| Eip-1F | Intergenic | CTGTGATCTTCCGCCAAAAT |
| Eip-1R | Intergenic | GCCTGAGAAATGGCTGAAAA |
| Eip-2F | yicL | CAATTCCTCTCACCGACGAT |
| Eip-2R | eipB | GGAACTGCAGCAAGAAGACC |
| Eip-3F | eipB | GAATTATGTCGGCACCGATT |
| Eip-3R | eilA | TTCGGTACGAAAGGGAAAAA |
| Eip-4F | eilA | AGGCGATGCGATAGTTTTTGAT |
| Eip-4R | eaeX | CTGATTGGTACTGGCAATCGTC |
| Eip-5F | eaeX | CGCCGACCATGTAGCAGTATAA |
| Eip-5R | eaeX | GGAGCTGTGTTGGCTTATTCCT |
| Eip-6F | eaeX | CGTAAAGGTGACGGTCTGCTC |
| Eip-6R | yicM | GGCAATGTTTGCCAGTTTGT |
| Eip-7F | yicM | CGCCGCATTAAAGACATTG |
| Eip-7R | yicP | ATTATGGCTGTCATGGCTGA |