FIG. 2.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of YcfN and YloS overexpression. Lane 1, total protein from the cell extract of the YcfN overexpression strain before induction; lane 2, total protein from the cell extract of the YcfN overexpression strain after induction; lane 3, soluble protein from the cell extract of the YcfN overexpression strain after induction; lane 4, total protein from the cell extract of the YloS overexpression strain before induction; lane 5, total protein from the cell extract of the YloS overexpression strain after induction; lane 6, soluble protein from the cell extract of the YloS overexpression strain after induction; lane 7, YloS after purification on a HisBind column.