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. 2004 Jun;186(11):3499–3507. doi: 10.1128/JB.186.11.3499-3507.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 4. — ADP · AlF_x-dependent complex formation between the DctD AAA+ domain and σ⁵⁴. Complex formation between DctD proteins and native S. enterica serovar Typhimurium σ⁵⁴(A), histidine-tagged S. enterica serovar Typhimurium σ⁵⁴ (B), or N-terminal histidine-tagged S. meliloti σ⁵⁴ (C and D) was assessed. DctD_(141-390), DctD_(141-394), and a histidine-tagged version of DctD₍_Δ_1-142) were included in binding reaction mixtures as indicated at the bottom of each lane. Protein complexes were resolved on a native gel and visualized by silver staining. Arrows in panels A to C indicate the complexes formed between σ⁵⁴ proteins and the DctD AAA+ domain proteins. No complexes were detected between σ⁵⁴ and DctD₍_Δ_1-142) under the same reaction conditions. Panel A shows binding reactions done with S. enterica serovar Typhimurium σ⁵⁴, panel B show reactions with the histidine-tagged S. enterica serovar Typhimurium σ⁵⁴, and panels C and D show reactions with the histidine-tagged S. meliloti σ⁵⁴.