TABLE 1.
Phenotypes displayed by wild-type isolates and isogenic flagellar protein export and filament mutants
| Background strain | Relevant characteristic | FlaA synthesis | Filament assembly | Motility | Secretion | Adherencea | Invasionb | I/Ac |
|---|---|---|---|---|---|---|---|---|
| F38011 | Wild type | + | + | + | + | 100 ± 5 | 100 ± 20 | 5.3 |
| flhB | − | − | − | − | 47 ± 9 | 0.24 ± 0.04 | 0.03 | |
| flgB | − | − | − | − | 40 ± 6 | 0.46 ± 0.1 | 0.06 | |
| flgC | − | − | − | − | 73 ± 2 | 2.1 ± 0.5 | 0.13 | |
| flgE2 | − | − | − | − | 13 ± 2 | 0.26 ± 0.1 | 0.11 | |
| fliD | + | + | + | + | 59 ± 8 | 44 ± 4 | 4.0 | |
| ciaB | + | + | + | − | 223 ± 15 | 1.9 ± 0.7 | 0.02 | |
| 81116 | Wild type | + | + | + | + | 100 ± 9 | 100 ± 27 | 2.7 |
| flaA flaB | − | − | − | − | 191 ± 27 | 10 ± 3 | 0.14 | |
| flaA | − | Truncated | − | + | 145 ± 19 | 70 ± 7 | 1.3 | |
| flaA flaB/pMEK3502 (flaA+) | + | + | + | + | 127 ± 18 | 29 ± 1 | 0.63 | |
| flaA flaB pMEK3503 (flaB+) | − | Truncated | − | + | 255 ± 27 | 43 ± 7 | 0.46 |
a
As a percentage of the wild-type value, which was 9.4 × 105 ± 0.5 × 105.
b
As a percentage of the wild-type value, which was 5.0 × 104 ± 1 × 104.
c
I/A, [(number of internalized bacteria)/(number of adherent bacteria)] × 100.