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. 2004 Jun;186(11):3408–3414. doi: 10.1128/JB.186.11.3408-3414.2004

FIG. 1.

FIG. 1.

SDS-polyacrylamide gel electrophoresis and Western blotting of purified recombinant C. aurantiacus SOD. (A) The protein fraction was isolated from E. coli ER2508 expressing C. aurantiacus SOD as the MBP-SOD fusion protein. MBP-SOD was isolated by using an amylose column. Lane 1, protein ladder; lane 2, E. coli whole-cell extract with MBP-SOD; lane 3, amylose column flowthrough; lane 4, pure MBP-SOD eluted from the amylose column. (B) Pure SOD was obtained by factor Xa cleavage of MBP-SOD and was isolated by IEF. Lane 1, protein ladder; lane 2, pure recombinant SOD. (C) Western blot with anti-wtSOD antibodies. Lane 1, prestained protein ladder; lane 2, C. aurantiacus whole-cell extract; lane 3, pure recombinant SOD.