FIG. 2.
Runoff transcription and EMSA of HMtA2 binding. (A) Electrophoresis of the 225-nt 32P-labeled runoff transcripts synthesized in 30 min at 58°C on templates preincubated with HMtA2 as indicated (dimers per 100 bp) above each lane. The amount of transcript, as a percentage of that synthesized in the absence of HMtA2 (−) is listed below each lane. Lane S contained size standards. (B) Autoradiogram of the electrophoretic separation of the complexes formed by incubation of 32P-labeled template DNA (50 ng) without (−) and with HMtA2 at the histone dimer/100-bp ratio indicated above each lane.