FIG. 2.
Expression of yggA-lac in pHYD956 transformants of the wild-type (WT) strain MC4100 and the following mutant derivatives: argR, GJ4748; argR yggA, GJ4894; argR argP, GJ4895; and argPd, MC4100/pHYD926. Cultures were grown to exponential phase in glucose-minimal medium supplemented with trimethoprim and additives (each at 1 mM, except for CAN, which was added to 40 μg/ml), as indicated in the inset key, for β-galactosidase assays. Enzyme specific activity values are given in Miller units (33). Cit, citrulline.