TABLE 1.
List of E. coli K-12 strainsa
| Strain | Genotypeb |
|---|---|
| MC4100 | Δ(argF-lac)U169 rpsL150 relA1 araD139 flbB5301 deoC1 ptsF25 |
| SK2226 | Δ(gpt-proA)62 trpE3 hisG4 galK2 xylA7 lac mtl-1 glnV44 ΔargH1 zif-290::Tn10 |
| GJ4536 | MC4100 argP202::λplacMu55(Kan) |
| GJ4674 | zbh-900::Tn10dKan(Ts)1 lacZ4525::Tn10dKan lon-103::IS186 serA |
| GJ4676 to GJ4682c | serA+argO::Tn10dTet derivatives of GJ4674 |
| GJ4748 | MC4100 argR64 zhb-914::Tn10dCm |
| GJ4822 | MC4100 argO204::Tn10dTet |
| GJ4894 | GJ4748 argO204::Tn10dTet |
| GJ4895 | GJ4748 argP202::λp(lacZ::Tn10dTet)Mu-Kan |
a
Strain MC4100 (20) was described earlier, and SK2226 was obtained from the E. coli Genetic Stock Center. The other strains were obtained in this study.
b
Genotype designations are as described in the work of Berlyn (4). As described in the text, argO is the new designation proposed for the anonymous open reading frame denoted yggA in the work of Blattner et al. (5), and the two have been used interchangeably in this paper.
c
Strains GJ4676 to GJ4682 are further described in Table 4.