TABLE 3.
Molecular analysis of plasmid-borne mutations in argP conferring Canr in argP202 strain GJ4536
| Plasmid | Amino acid change (codon alteration)a | Dominance of mutationb |
|---|---|---|
| pHYD926c | S94L (TCA→TTA) | D |
| pHYD927 | P108S (CCT→TCT) | D |
| pHYD928 | V144M (GTG→ATG) | D |
| pHYD929 | P217L (CCC→CTC) | D |
| pHYD930 | L294F (CTT→TTT) | D |
| pHYD931 | R295C (CGT→TGT) | R |
| pHYD932 | A68V (GCA→GTA) | D |
a
The reference argP (iciA) sequence (297 sense codons) and annotation are from the work of Blattner et al. (5) (GenBank accession number AE000375). Amino acid alterations are given in a one-letter code. Mutated nucleotides in the corresponding codons are marked in bold.
b
The mutation was classified as dominant (D) if the corresponding plasmid conferred Canr in MC4100 (argP+) and as recessive (R) if it did not.
c
Plasmid pHYD926 also carries a silent synonymous codon substitution mutation (GGG→GGA) in the penultimate codon of the divergently transcribed open reading frame yqfE situated upstream of argP (see Fig. 2).