TABLE 4.

Molecular analysis of Tn10dTet insertions in yggA

Strain	yggA (argO) allelea	Characteristics of Tn10dTet insertion		Site of YggA truncationd
		Coordinates of 9-bp duplicated regionb	Orientationc
GJ4676	204	8926-8934	I	V77
GJ4677	205	9046-9054	II	I37
GJ4678	206	8926-8934	II	V77
GJ4679	207	9010-9018	I	V49
GJ4680	208	9009-9017	II	V49
GJ4681	209	8923-8931	I	A78
GJ4682	210	9170-9178	II	—e

^aBased on the results described in this paper, yggA has been redesignated argO.

^bThe coordinates of the 9-bp host sequence duplication after Tn10dTet insertion (25, 33), inferred from determination of the junction sequence at the counterclockwise end of the insertion, are given. The coordinates are based on the work of Blattner et al. (5) (GenBank accession number AE000375), according to which the yggA open reading frame extends from bp 9156 to 8521 on the complementary strand (211 sense codons).

^cOrientation I is that in which the sequence (in the Tn10dTet insertion) for primer TetR is counterclockwise to that for primer TetF, and the reverse is true for orientation II.

^dThe residue number and identity (in one-letter code) of the terminal amino acid upstream of the insertion are indicated.

^e—, the argO210 insertion is situated 22 bp upstream of the yggA open reading frame.