TABLE 1.

Primers used in this study

Primer	Sequencea	Positionb	Primer use
A	−5′ GTATGGGATGCCACTCAGCTCAAAC 3′	378-354	Inverse PCR
B	+5′ TGCCGGAAGCAGCTTTGGAATTCAC 3′	567-591	Inverse PCR
C	+5′ AAGGCATATCGAGCCATGAACTGCA 3′	867-892	Inverse PCR
D	−5′ AGCCGTCAAAGGACTCCATCCTGTG 3′	613-591	Inverse PCR
LigBamHI	+5′ CTGGATCCAGTGGTGAATGAGCTGG 3′	−55-−31	ddlc. innocuum cloning
LigSalI	−5′ GCTTGTCGACGGAATCAGCTTCTG 3′	1178-1154	ddlc. innocuum cloning
RacBamHI	+5′ CCGTTTCCGGATCCGATTGACAAG 3′	1018-1041	C. innocuum racemase cloning
RacSall	−5′ ATTGTCGACCTTCTCTTGAAAAATAG 3′	2265-2240	C. innocuum racemase cloning
XYSacII	+5′ TTGAGAGCTCTGGCAGAGGAG 3′	−28-−9	vanXYc cloning
XYBamHI	−5′ GTTCGCATAATAAATAAAGGATCCGA 3′	589-564	vanXYc cloning

^aRestriction sites introduced in the primer sequence are underlined; +, direct primer; −, reverse primer.

^bPosition relative to the ATG start codon in the ddl_{c. innocuum} or in the vanXY_c genes.