TABLE 3.

Peptidoglycan precursors in extracts of E. faecalis JH2-2 harboring various plasmids and for which MICs of vancomycin differ

Plasmid	MIC of vancomycin (μg/ml)	Type of peptidoglycan precursors (%)a
		Tetra	Penta[Ser]	Penta[Ala]
pJIM2246	2	—b	—	100
pJIM2246Ω ddlc. innocuum	2	—	—	100
pJIM2246Ω C. innocuum racemase gene	2	—	—	100
pJIM2246Ω ddlc. innocuum, C. innocuum racemase gene	2	—	9	91
pJIM2246Ω vanXYc	2	90.8	—	9.2
pJIM2246Ω ddlc.innocuum, vanXYc	2	NDc	ND	ND
pJIM2246Ω C. innocuum racemase gene, vanXYc	2	92.5	—	7.5
pJIM2246Ω ddlc. innocuum, C. innocuum racemase gene, vanXYc	6	28.5	11.7	59.8

^aTetra, UDP-MurNac-l-Ala-γ-d-Glu-l-Lys-d-Ala; Penta[Ser], UDP-MurNac-l-Ala-γ-d-Glu-l- Lys-d-Ala-d-Ser; Penta[Ala], UDP-MurNac-l-Ala-γ-d-Glu-l-Lys-d-Ala-d-Ala.

^b—, not detected.

^cND, not done.