Skip to main content
. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Eur J Nucl Med Mol Imaging. 2008 Jul 26;35(12):2275–2285. doi: 10.1007/s00259-008-0870-6

Fig. 2.

Fig. 2

a To a buffer solution with rising amounts of FL concentration and fixed amounts of ATP and MgSO4, fixed amounts of 14C-D-luciferin or D-luciferin were added, and the bioluminescence signal was measured. Light signal was detected by the luminometer from an amount of 8 ng FL on. The detected amount of light signal is displayed on the y-axis and given in relative light units (RLU) per minute. There were no significant differences in the measured light yields when using 14C-D-luciferin in comparison to unlabeled luciferin (p=0.36). b To test the functionality of 14C-D-luciferin in cell culture, 293T cells expressing FL and native cells were investigated. After adding corresponding amounts of unlabeled or 14C-labeled D-luciferin to the wells, light emission was measured in a CCD camera. Up to 173 min after adding the substrate to the FL-expressing 293T cells, we found slightly lower light levels (p=0.02) in wells with labeled D-luciferin, at time point 720 min, no significant differences were observed (p=0.2)