Skip to main content
. Author manuscript; available in PMC: 2015 Sep 4.
Published in final edited form as: Mol Cell. 2014 Aug 14;55(5):708–722. doi: 10.1016/j.molcel.2014.07.005

Figure 3. NF-Y binds to and regulates core ESC self-renewal and pluripotency genes.

Figure 3

(A–B) Genome browser shots showing NF-Y co-occupancy with master ESC TFs (Chen et al., 2008; Ma et al., 2011; Marson et al., 2008) at the enhancers of ESC identity genes (A), and at the promoters/enhancers of differentiation/developmental genes (B).

(C) Western blot analysis of NF-YA, NF-YB, and NF-YC in NF-YA knockdown (KD), NF-YB KD, NF-YC KD, or NF-Y triple KD (TKD; KD of all three NF-Y subunits) ESCs 96h after siRNA transfection. Ran is used as a loading control.

(D) RT-qPCR analysis of relative mRNA levels of ESC identity genes (left), and differentiation markers (right) in NF-YA KD, NF-YB KD, NF-YC KD, and NF-Y TKD ESCs compared to control KD ESCs 96h after siRNA transfection. Data normalized to Actin, HAZ, and TBP. Error bars, SEM. See also Figure S2.