TABLE 9.

Effect of GlutDH overexpression on gltA expression in the tnrA⁺ background^a

Strain	Relevant genotype	β-Galactosidase activity (gltA-lacZ fusion) (U) in medium with glucose plus:
		Ammonium	Glutamate
BB1281	rocG	68.8	2.5
BB1310	rocG gudB1	122.2	23.2
BB1318	rocR(T120I)	51.5	2.6
BB1749	rocGp1	59.8	2.2
BB1793	rocR(T120I) rocGp1	57.2	82.4

^aAll strains have a gltA-lacZ fusion as in LG219. Strains BB1281 and BB1749 contain the ΔgudB::tet mutation, which prevents the emergence of spontaneous gudB1 mutants. Strain BB1793 contains the yweA::pBB1013 (′yweA′ neo) insertion linked to the rocGp1 allele. The strains were grown to mid-exponential phase in the indicated minimal glucose media and assayed for β-galactosidase activity.