Table 1.

Time line of data collection and stressor application. All cats in the study were subjected to events on the same day.

Day 1	Day 1 baseline SB data collection
Day 10	Day 10 baseline SB data collection
Day 11	Baseline blood collection
Day 18- application of stressors begins	Day 1- no EE (removal of food treats, social interactions with JS, auditory EE, and playtime out of cage), new caretaker #1, change in husbandry and feeding time (from AM to PM)
Day 19	Day 2- no EE(removal of food treats, social interactions with JS, auditory EE, and playtime out of cage), new caretaker #2, change in husbandry and feeding time (from AM to PM)
Day 20	Day 3- no EE(removal of food treats, social interactions with JS, auditory EE, and playtime out of cage), new caretaker #3, change in husbandry and feeding time (from PM to AM)
Day 21	Day 4-no EE(removal of food treats, social interactions with JS, auditory EE, and playtime out of cage), food removed in PM
Day 22- application of stressors ends	Day 5- no EE(removal of food treats, social interactions with JS, auditory EE, and playtime out of cage), restraint in carrier, anesthesia, blood draw
Day 29	End of stress period data collection