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. 2014 Mar 29;1(5):307–318. doi: 10.1002/acn3.47

Figure 4.

Figure 4

IL-33 directly induces expression of MS related genes. (A) PBMC isolated from healthy controls were cultured with or without IL-33 (10 ng/mL) for 48 h. RNA was isolated from cultures followed by cDNA synthesis and quantitative RT-PCR to determine transcript levels of the indicated genes. Expression of individual genes was normalized to GAPDH after PBMC were cultured. Results are expressed as fold induction by IL-33 relative to untreated cultures, mean of four experiments. Filled columns; P < 0.01, open columns; P > 0.05. (B) PBMC were cultured with no stimulus or with anti-CD3, the indicated TLR agonists, lymphotoxin alpha, TNF-alpha + IFN-alpha, or TFF2 for 24–72 h. After harvest, RNA purification and cDNA synthesis, the expression level of IL-33 was determined by quantitative PCR. Results are expressed as transcript levels after normalization to GAPDH, mean of three experiments. *P < 0.01.