TABLE 1.
B. subtilis strains used
| Strain | Genotype | Source or reference |
|---|---|---|
| SMY | Wild type | P. Schaeffer |
| BR151MA ccpA::spc | ccpA::[(Tn917-lacZ)::spc] trpC2 lys-3 | T. Henkin |
| GM1222 | ptsH1 ykwC::pJH101 (′ykwC′ cat) trpC2 sacB′-lacZ | 12 |
| QB5618 | rocD::aphA3 trpC2 ΔamyE::Φ(rocD′-lacZ cat) | 17 |
| QB5626 | rocR(T120I) trpC2 ΔamyE::Φ(rocD′-lacZ cat) | 17 |
| QB7096 | crh::aphA3 trpC2 | 27 |
| SG82 | lacA::tet trpC2 | 10 |
| BB1179 | ccpA::[(Tn917-lacZ)::spc)] | SMY × DNA (BR151MA ccpA::spc) |
| BB1236 | rocD::aphA3 | SMY × DNA (QB5618) |
| BB1264 | rocG::pBB907 (′yweA rocG rocA′) neo | SMY × pBB907 |
| BB1271 | ΔrocG::ble ΔgudB::teta | 7 |
| BB1316 | rocR(T120I) | BB1236 × DNA (QB5626) |
| BB1434 | rocG::pBB923 [Φ(rocG′p+-lacZ erm)] | 5 |
| BB1512 | ΔamyE::Φ(DAS-rocG′p+-lacZ erm) | SMY × pBB996 (5) |
| BB1537 | ΔrocG::ble ΔgudB::tet ccpA::[(Tn917-lacZ)::spc] | BB1271 × DNA (BB1179) |
| BB1575 | ptsH1 ykwC::pJH101 (′ykwC′ cat) | SMY × DNA (GM1222) |
| BB1597 | rocR(T120I) ccpA::[(Tn917-lacZ)::spc] | BB1316 × DNA (BB1179) |
| BB1628 | yweA::pBB1013 (′yweA′ neo) | SMY × pBB1013 |
| BB1744 | rocGp1 ΔgudB::tet | BB1271 × pBB1048 |
| BB1770 | rocGp1 yweA::pBB1013 (′yweA′ neo) ΔgudB::tet | BB1744 × pBB1013 |
| BB1792 | rocGp1 yweA::pBB1013 (′yweA′ neo) rocR(T120I) | BB1316 × DNA (1770) |
| BB2062 | rocG::pBB1096 [Φ(rocG′p1-lacZ erm)] | SMY × pBB1096 |
| BB2063 | ΔamyE::Φ(DAS-rocG′p1-lacZ erm) | SMY × pBB1049 |
| BB2163 | ccpA::[(Tn917-lacZ)::spc] yweA::pBB1013 (′yweA′ neo) | BB1628 × DNA (BB1179) |
a
The ΔgudB::tet null mutation in the gene coding for the inactive GudB-GlutDH was present in some strains and served to prevent spontaneous gudB1 mutations from allowing expression of active GudB1-GlutDH (7).