TABLE 3.

Activity of rocG-lacZ fusions^a

Strain	Relevant genotype	Promoter region within the fusion	β-Galactosidase activity (U)
			Medium with glucose plus:				Medium without glucose but with:
			NH4Cl	Glutamate	Proline	Ornithine	Proline	Ornithine
BB1434	Wild type	rocGp+	0.39	0.47	0.49	0.52	2.3	11.3
BB1439	ccpA	rocGp+	1.1	1.6	1.2	11.7	3.2	16.8
BB1470	ccpA rocR(T120I)	rocGp+	NGb	6.2	7.1	14.2
BB2155	lacA	rocGp+	0.15	0.10	0.54
BB2157	lacA ccpA	rocGp+	0.54	0.32	18.3
BB2062	Wild type	rocGp1	1.9	1.0	13.9	11.5
BB2156	lacA	rocGp1	2.8	1.2	19.9
BB2158	lacA ccpA	rocGp1	0.48	0.28	12.4

^aAll strains are derivatives of BB1434 or BB2062 and contain a rocG-lacZ fusion integrated at the rocG locus. Cells were grown in minimal medium with the indicated carbon and nitrogen sources and assayed for β-galactosidase activity.

^bNG, no growth under these conditions.