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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Nat Cell Biol. 2013 Aug 18;15(10):1176–1185. doi: 10.1038/ncb2829

Figure 6. Riquiqui and Minibrain function downstream of Dachsous to promote Yorkie activity.

Figure 6

Wing imaginal discs from third instar D. melanogaster larvae harbouring the following transgenes: (a) hh-Gal4, UAS-ds and ex-lacZ; (b) hh-Gal4, UAS-ds, UAS-riqRi and ex-lacZ; (c) hh-Gal4, UAS-ds, UAS-mnbRi and ex-lacZ; (d) hh-Gal4, UAS-ftΔICD and ex-lacZ; (e) hh-Gal4, UAS-ftΔICD, UAS-riqRi and ex-lacZ. Yki activity was reported by ex-lacZ levels (grey in a-e). Cubitus interruptus (Ci) expression (red in a’-e’) marked the anterior compartment of wing imaginal discs. Merged images are shown in (a’’-e’’). X-Z sections through the wing pouch are shown below planar sections of wing discs in a-c”. (f) Levels of ex mRNA and Diap1 mRNA relative to the control actin5C mRNA, assessed by QRT-PCR, in wing imaginal discs harbouring the following transgenes: 32B-Gal4 and UAS-ftΔICD; and 32B-Gal4, UAS-ftΔICD and UAS-riqRi. Data is presented as mean ± SEM, n=3, ** = p<0.01.