TABLE 2.

Transcription analysis of miaA and hfq by using lacZ reporter fusions^a

lacZ fusionb	β-Galactosidase activitiesc
	BHI medium		BHI medium + 4% NaCl
	Wild-type strain	ΔsigB strain	Wild-type strain	ΔsigB strain
miaA-hfq-lacZ (−1125 and 107)	21.5 ± 0.2	20.0 ± 0.5	39.9 ± 0.2	21.4 ± 0.5
miaA-lacZ (−1125 and −109)	178.9 ± 3.5	172.4 ± 7.5	138.4 ± 3.2	144.8 ± 8.1
hfq-lacZ (−109 and 107)	0.65 ± 0.05	0.65 ± 0.04	18.1 ± 0.1	0.65 ± 0.04

^aThe wild-type and ΔsigB strains containing the lacZ fusions were grown in BHI medium until the OD₆₀₀ was 0.3. The cultures were split, and NaCl was added to one-half of each culture at a final concentration of 4%. After 1 h, cell pellets were harvested and subjected to β-galactosidase assays.

^bThe numbers in parentheses are the starting and ending positions of the fragment fused to lacZ relative to the hfq transcription start site (position 1).

^cβ-Galactosidase activities were determined as described in Materials and Methods.