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. 2014 Jul 19;65(18):5243–5255. doi: 10.1093/jxb/eru288

Fig. 3.

Fig. 3.

Phenotype of SlNCED1/2/3-RNAi-treated fruits. At the maturation green stage, 48 tomato fruits (cv. Tom) from 10 independent plants grown in the greenhouse were selected and divided into four groups (each group has 12 fruits for three repetitions; each repetition has four fruits) for VIGS testing. The SlNCED1-RNAi, SlNCED2-RNAi, and SlNCED3 -RNAi TRV vectors were injected from the pedicel into group 1, group 2, and group 3, respectively. Group 4 was injected with TRV vector (no NCED gene) as a control. The fruits were evaluated 3–12 d after inoculation. Panels A to F show Tom tomato fruits 9 d after VIGS treatments: (A) Control; (B) SlNCED2-RNAi-treated fruit; (C) SlNCED3-RNAi-treated fruit; (D–F) SlNCED1-RNAi-treated fruit. Panels G–N show JiaBao tomato fruits treated with SlNCED1-RNAi: (G) MG stage fruit (before treatment); (H) 5 d days after inoculation (DAI); (I) 7 DAI; (J) 9 DAI; (K, L) 12 DAI; (M, N) 12 days after MG stage, control fruit.