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Effect of changes in potential zinc finger residues on Q activity
| Amino acidsubstitutiona | In vivo activity (% WT λQ)b |
|---|---|
| C118S | 9.2 |
| C121S | 8.9 |
| C144S | 9.1 |
| C147S | 7.6 |
| C118H | 8.0 |
| C121H | 7.1 |
| C144H | 8.0 |
| C147H | 8.4 |
| C121A | 6.5 |
| C53S | 50.0 |
| C108S | 85.0 |
| C191A | 86.4 |
Codon changes are TGC(Cys)→AGC(Ser), TGC(Cys)→CAC(His), and TGC(Cys)→ GCC(Ala).
WT, wild type.
