FIG. 1.

In vitro transcription to examine the effects of the CIRCE element and recombinant HrcA: structure of a pair of promoter constructs and results of an in vitro transcription reaction performed with C. trachomatis RNA polymerase in the absence (minus sign) or presence (plus sign) of 1,150 nM HrcA. (A) The wild-type dnaK promoter (upper line), containing a CIRCE element, was transcribed with the omcB promoter (lower line), and these promoters produced 153- and 125-nucleotide transcripts, respectively. w.t., wild type. (B) The dnaK promoter containing the hctA upstream region (upper line) and thus lacking the CIRCE element was transcribed with the hctA promoter containing the dnaK upstream region and its CIRCE element (lower line).