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In vitro transcription with C. trachomatis and E. coli RNA polymerases. The upper band is the transcript from the dnaK promoter, and the lower band is the transcript from the omcB promoter. Lane 1, C. trachomatis RNA polymerase in the absence of HrcA; lane 2, C. trachomatis RNA polymerase and 1,150 nM HrcA; lane 3, E. coli RNA polymerase in the absence of HrcA; lane 4, E. coli RNA polymerase and 1,150 nM HrcA.
