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DNase I footprint of recombinant HrcA on the dnaK CIRCE region. (A) Each lane contained approximately 10,000 cpm of labeled dnaK probe. Lane 1, G sequencing ladder; lane 2, G-A sequencing ladder; lane 3, no HrcA; lane 4, 144 nM HrcA; lane 5, 288 nM HrcA. (B) Sequence of dnaK CIRCE region. Protected regions are underlined, and DNase-hypersensitive sites are indicated by vertical arrows.
