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Targeted PCR amplification of TaTEF-7A in T. aestivum cv. Chinese Spring, the nullisomic–tetrasomic lines for homoeologous group 7, and three putative diploid progenitors of wheat with genome-specific primer sets. (A) Primer TaTEF-1F/R; (B) primer TaTEF-2F/R; (C) primer TaTEF-3F/R. M, marker; 1, T. urartu; 2, Ae. speltoides; 3, Ae. tauschii; 4, T. aestivum; 5, N7AT7B; 6, N7AT7D; 7, N7BT7A; 8, N7BT7D; 9, N7DT7B; 10, N7DT7A; 11, H2O.
