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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: J Mol Biol. 2012 Feb 24;421(0):364–377. doi: 10.1016/j.jmb.2012.02.026

Figure 1.

Figure 1

Sedimentation velocity analysis of apoC-II fibrils formed at different apoC-II concentrations. ApoC-II samples were prepared by incubating apoC-II in refolding buffer for 14 days prior analytical ultracentrifugation at 8,000 rpm at 20°C with radial scans taken at 8 minute intervals. Panel A: Radial scans for apoC-II sample (0.3 mg/mL), monitored at 280 nm (open circles). The fit of the data to a c(s) model45 is also shown (solid lines). Panel B: Sedimentation coefficient distributions for fibril samples formed at 0.1 mg/mL (dotted line), 0.3 mg/mL (solid line) and 0.5 mg/mL (dashed line). Panel C: Dependence of the weight-average sedimentation coefficient on total apoC-II concentration for the faster moving and slower moving population of fibrils (closed circles and closed squares, respectively).