Table 1.
Characterization of free pool and closed-loop fraction in apoC-II fibril samples
| Total apoC-II (mg/ml) | Non-sedimenting apoC-II (mg/ml) | Closed loops (mg/ml) |
|---|---|---|
| 0.1 | 0.0193 | 0.0099 |
| 0.2 | 0.0195 | 0.0157 |
| 0.3 | 0.0197 | 0.0197 |
| 0.4 | 0.0231 | 0.0198 |
| 0.5 | 0.0257 | 0.0193 |
| 0.6 | 0.0264 | a |
ApoC-II samples were prepared by incubating apoC-II in refolding buffer for 14 days prior to sedimentation velocity analysis. The concentration of non-sedimenting apoC-II was determined from the optical density in the supernatant at the conclusion of centrifugation.
a
A closed-loop population was not observed for fibrils formed at 0.6 mg/ml.