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. 2004 Jun;186(11):3480–3491. doi: 10.1128/JB.186.11.3480-3491.2004

FIG. 2.

FIG. 2.

(A) Zymogram assay to investigate any possible cell binding capabilities of Tal2009. Lanes 1 and 2 show a zone of lytic activity corresponding to the Tuc2009 endolysin in the supernatant and cell pellet, respectively, following incubation with 509.9 cells. Lane 3 shows that Tal2009 is in the supernatant, while lane 4 shows Tal2009 to be absent from the 509.9 cell pellet. The gels contained 0.4% (wt/vol) L. lactis UC509.9 autoclaved cells. Molecular masses are indicated. (B) Zymogram analysis of the E. coli M15 lysates containing pTal2009-2ΔN or the mutant pTal2009mut-2ΔN in gels containing 0.4% (wt/vol) L. lactis UC509.9 autoclaved cells. Lane 1 contains the negative control pQE60, and lanes 2 and 3 contain pTal2009-2ΔN and pTal2009mut-2ΔN, respectively. Molecular masses are indicated to the left of the gel.