FIG. 3.

Western blot to detect possible interaction between Tal₂₀₀₉-Δ5N and Tal₂₀₀₉ΔC. The negative control is in lane 2, and the lysates of E. coli cultures overexpressing protein from pTal₂₀₀₉ΔC and pTal₂₀₀₉-5ΔNtet are in lanes 3 and 4, respectively. Lysates resulting from concomitant induction of expression from pTal₂₀₀₉-5ΔNtet and pTal₂₀₀₉ΔC can be observed in lane 5, with a band of ∼30 kDa denoted by an arrow. Lane 1 was loaded with a six-His protein ladder which reacts to the commercial Abs, and the molecular masses are indicated.