TABLE 2.
List of oligonucleotides used in the construction of plasmids
| Oligonucleotide name | Oligonucleotide sequencea |
|---|---|
| Tal2009-1F | 5′ AAA CCA TGG GTA ATA TCT TAT TTT TAG ATA AG |
| Tal2009-2F | 5′ AAA CCA TGG GCA ATA TCT CTG ACC TTG |
| Tal2009-3F | 5′ AAA CCA TGG CAG ATT TTA TAA ATG CAG |
| Tal2009-4F | 5′ AAA CCA TGG TCT CTA AAC AAG CGG CG |
| Tal2009-5F | 5′ AAA CCA TGG CCT TAA ACG GAC ACC CTG AAC G |
| Tal2009-6F | 5′ AAA CCA TGG CAA GTG AAA TGG GTT GG |
| Tal2009mut-2ΔNF | 5′ GGG CGA AGC TCT CGC CGC G |
| Tal2009mut-2ΔNR | 5′ CGC GGC GAG AGC TTC GCC C |
| Tal2009-R | 5′ GGA AGA TCT AAA TTT GAT ATA ATC CCT TGG ATT C |
| Tal2009-RΔC | 5′ GGA AGA TCT CGC ATG CTT GAT GAC CGT G |
| Lys F | 5′ AAA CCA TGG AAA GAT TAA TCA AAA AAT C |
| Lys R | 5′ GGA AGA TCT ATA ATT TAG TGT TTG ACC AGC |
| TetKF | 5′ GGA ATT C[CA TAT G]GC TTC ACA GAA ATT CTA GAA CA |
| TetKR | 5′ GGA ATT C[CA TAT G]GT TAA TAC GTG AGC TCT GCG AGG C |
a
NcoI and BglII sites are underlined once and twice, respectively. NdeI sites are in brackets.