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Relative level of light emission by rei2 and its mutant derivativesa
| Strain | Relative light units ± SD
|
Increase (fold) | |
|---|---|---|---|
| −Lys | +Lys | ||
| rei2 | 5 ± 0.1 | 20 ± 0.2 | 4 |
| rei2-6 | 5 ± 0.1 | 6 ± 0.1 | 1.2 |
| rei2-7 | 5 ± 0.1 | 7.5 ± 0.1 | 1.5 |
Bacterial cells were grown on M9 minimal medium with citrate as the carbon source without (−) and with (+) 5 mM lysine (Lys) for 3 h at 30°C. Light emission was then determined in a luminometer as described in Materials and Methods.
