TABLE 2.
Induction of the davD promoter in the wild-type P. putida KT2440 and its isogenic mutant KT2440 mut-1 and RpoNa
| Strain | Activity (mean Miller units ± SD) on substrate
|
||||
|---|---|---|---|---|---|
| None | Lysine | Cadaverine | Aminovalerate | Glutarate | |
| KT2440 | 620 ± 10 | 1,280 ± 100 | 930 ± 60 | 1,730 ± 20 | 600 ± 10 |
| mut-1 | 470 ± 10 | 530 ± 30 | 840 ± 10 | 1,700 ± 120 | 580 ± 20 |
| RpoN | 620 ± 40 | 1,450 ± 30 | 1,370 ± 100 | 1,800 ± 50 | 550 ± 10 |
a
Wild-type, mut-1, and RpoN strains bearing pOR2 were grown in M9 minimal medium with benzoate as the C source. Where indicated, cultures were supplemented with 5 mM lysine, 2.5 cadaverine, 5 mM δ-aminovalerate, and 5 μM glutarate. The β-galactosidase activity was determined 7 h later in cells growing exponentially. The activity is given in Miller units, and values are averages of three to six independent determinations.