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. 2014 Sep 8;9(9):e105912. doi: 10.1371/journal.pone.0105912

Figure 7. Phosphorylation-deficient stathmin attenuates HGF protective effects against thrombin-induced permeability and cytoskeletal remodeling.

Figure 7

Endothelial monolayers transfected with phosphorylation-deficient stathmin (STMN-S63A) or empty vector (Em. Vec.) were treated with thrombin or HGF (50 ng/ml) + thrombin (0.5 U/ml). A: TER measurements were performed over 1.5 hrs. Results are representative of four independent experiments. Bar graphs depict results of quantitative analysis of TER data; *P<0.05. B-C: F-actin remodeling (B, bar  =  5 µm) and MT remodeling (C, bar  =  10 µm) in EC expressing STMN-S63A was performed by double immunofluorescence staining with either Texas Red phalloidin, or β-tubulin antibody and His-tag antibody to detect STMN-S63A expressing cells. The magnified images (insets) show the details of actin and MT structure in non-transfected and STMN-S63A expressing cells. Results are representative of three independent experiments. Bar graphs (C, lower right panel) depict results of quantitative image analysis of peripheral microtubules from three independent experiments; 10 cells from each experiment. *P<0.05.