Figure 3. Focused hepatic gene expression patterns in female Eastern mosquitofish (Gambusia holbrooki) using qualitative polymerase chain reaction analysis.

Gene expression of (A) vitellogenin (vtg), (B) zona pellucida glycoprotein 2 (zp2), (C) 17β-hydroxysteroid dehydrogenase 3 (17βhsd3) was analyzed between paper mill exposed (Fenholloway) and reference (Econfina) collection sites. Each point represents the mean of each group and error bars represent standard deviation; time points with no standard deviation have an N of 1. A box indicates statistical significance between groups at the selected oocyte developmental stages as determined using a Student's t-test with p<0.05.