TABLE 3.
Conserved domain search and COG comparison
| Gene | Deduced no. of residues | Functional assignment based on COG comparison | Pfam database search resultsa
|
||
|---|---|---|---|---|---|
| Conserved domain(s) | E-value | Residues | |||
| ORF3 | 398 | COG1960 (Acyl-CoA dehydrogenases) | Acyl-CoA dehydrogenase, N-terminal domain (pfam02771) | 2.4e-6 | 27-145 |
| Acyl-CoA dehydrogenase, middle domain (pfam02770) | 3.4e-16 | 147-248 | |||
| ORF4 | 435 | No hits | Amidohydrolase family (pfam04909) | 3.4e-08 | 41-373 |
| ditQ | 424 | COG2124 (cytochrome P450) | Cytochrome P450 (pfam00067) | 2.3e-24 | 39-421 |
| ditP | 140 | COG2128 (uncharacterized ancient conserved region) | No hits | ||
| ditO | 391 | COG0183 (acetyl-CoA acetyltransferase) | Thiolase, N-terminal domain (pfam00108) | 1.6e-86 | 1-260 |
| Thiolase, C-terminal domain (pfam0280) | 1.6e-63 | 265-389 | |||
| ditN | 301 | COG1250 (3-hydroxyacyl-CoA dehydrogenase) | 3-Hydroxyacyl-CoA dehydrogenase, NAD binding domain (pfam02737) | 2.3e-53 | 3-188 |
| 3-Hydroxyacyl-CoA dehydrogenase, C-terminal domain (pfam00725) | 5.8e-41 | 190-285 | |||
| ditM | 289 | COG0179 (2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [catechol pathway]) | Fumarylacetoacetate hydrolase (pfam01557) | 1e-45 | 86-254 |
| ditL | 359 | No hits | Amidohydrolase (pfam04909) | 3.6e-32 | 1-344 |
| ditK | 249 | No hits | Bacterial regulatory proteins, tetR family (pfam00440) | 2e-12 | 67-113 |
| ditJ | 546 | COG0318 (acyl-CoA synthetases [AMP-forming]/AMP-acid ligases II) | AMP-binding enzyme (pfam00501) | 7.5e-105 | 43-459 |
a
Pfam, Protein families database of alignments and HMMs.