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. 2014 Sep 8;9(9):e106724. doi: 10.1371/journal.pone.0106724

Figure 1. Schematic representation of the plasmid vector constructs tested for expression of mouse GIFT15 in E. coli.

Figure 1

1. pET20b: expression of GIFT15 without any tag, GM-CSF domain (lacking the signal peptide, green box) and IL-15 domain (blue box). The linker between the two domains is formed from the signal peptide and propeptide of IL-15 (grey box). 2. pET16b: expression of GIFT15 with an additional His-tag (6x-His) at the N-terminus of the fusokine. 3. pET32b: expression of GIFT15 downstream of thioredoxin (TRX, red box) followed by a His-tag. 4. pET42b: expression of GIFT15 in fusion with glutathione S-transferase (GST, yellow box). 5. pMALc4x: expression of GIFT15 in fusion with the maltose-binding protein (MBP, white box). 6. pHisMBP: expression of GIFT15 with the addition of a His-tag at the N-terminus of the MBP domain and replacement of the Factor Xa protease recognition site with that of the TEV protease.